Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: Expression of p62 and LC-3B in the whole kidneys of endotoxemic mice. (A) Renal p62 mRNA expression was detected in the whole kidneys of mice at various time points by reverse transcription-quantitative polymerase chain reaction analysis. (B) p62 and LC-3B protein expression was detected in the whole kidneys of mice at various time points by western blotting. (C) Densitometric analysis of p62 expression. (D) Densitometric analysis of LC-3B protein. n≥6. Data are presented as the mean ± standard deviation. p62, sequestome-1; LC-3B, light chain-3B.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: Localization of p62 protein in the renal cortex of mice during endotoxemia. (A) Immunostaining of kidney slides with anti-p62 and anti-IgG antibody in the renal cortex of normal controls (magnification, ×40). (B) The localization of p62 protein in the renal cortex of mice was measured by immunostaining at various time points (magnification, ×200). (C) Double-immunohistochemical staining of p62 and AQP-1 in consecutive kidney slides in normal mice (magnification, ×400). n≥6. IgG, immunoglobulin G; p62, sequestome-1; AQP-1, aquaporin-1.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Immunostaining, Immunohistochemical staining, Staining

Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: Localization of p62 protein in the outer medullas of mice during endotoxemia. (A) The localization of p62 protein in the outer medullas of mice was measured by immunostaining at various time points (magnification, ×200). (B) Double-immunohistochemical staining of NKCC-2 and p62 in consecutive kidney slides at 8 h following treatment with LPS (magnification, ×400). (C) Double-immunohistochemical staining of AQP-2 and p62 in consecutive kidney slides at 8 h following treatment with LPS (magnification, ×400). n≥6. Out, outer; p62, sequestome-1; NKCC-2, Na-K-2Cl cotransporter; AQP2, aquaporin-2; LPS, lipopolysaccharide.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Immunostaining, Immunohistochemical staining, Staining

Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: Localization of p62 protein in the inner medullas of mice during endotoxemia. (A) Localization of p62 protein in the inner medullas of mice was detected by immunostaining at various time points (magnification, ×200). (B) Double-immunohistochemical staining of p62 and AQP-2 in consecutive kidney slides at 12 h following treatment with LPS (magnification, ×400). (C) Double-immunohistochemical staining of p62 and AQP-1 on consecutive kidney slides at 12 h subsequent to treatment with LPS (magnification, ×400). n≥6. p62, sequestome-1; AQP, aquaporin; LPS, lipopolysaccharide.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Immunostaining, Immunohistochemical staining, Staining

Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: p62 protein expression in renal medullas of endotoxemic mice. p62 protein expression in renal medullas was detected in mice at various time points using (A) western blotting and (B) densitometric analysis. n≥6. Data are presented as the mean ± standard deviation. p62, sequestome-1.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: Effects of p62 overexpression on injury in renal tubular epithelial cells treated with LPS. (A) The expression of p62 protein in HK-2 cells transfected with control plasmid (pENTER) and p62 plasmid (pENTER-p62) following treatment with 1,000 ng/ml LPS for 12 h measured by western blotting (left panel). The right panel exhibits the ratios of p62/β-actin. (B) Cell viability of different groups measured using a Cell Counting Kit-8 kit (n=3). (C) LDH cytotoxicity in the different groups (n=3). (D) Ratios of cellular apoptosis (n=3). Data are presented as the mean ± standard deviation. p62, sequestome-1; LDH, lactate dehydrogenase; LPS, lipopolysaccharide.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Over Expression, Expressing, Transfection, Control, Plasmid Preparation, Western Blot, Cell Counting, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Dynamic expression and roles of sequestome-1/p62 in LPS-induced acute kidney injury in mice

doi: 10.3892/mmr.2018.8809

Figure Lengend Snippet: Effects of p62 interference on injury in renal tubular epithelial cells treated with LPS. (A) The levels of p62 expression in renal tubular epithelial cells transfected with control siRNA and siRNA-p62 following treatment with 1,000 ng/ml LPS for 12 h measured by western blotting (left panel). The right panel exhibits the ratios of p62/β-actin. (B) The viabilities of HK-2 cells in the different groups (n=3) measured using a Cell Counting Kit-8 kit. (C) LDH cytotoxicity detection in the different groups (n=3). (D) The ratios of apoptotic cells in the different groups (n=3). Data are presented as the mean ± standard deviation. p62, sequestome-1; LDH, lactate dehydrogenase; LPS, lipopolysaccharide; siRNA, small interfering RNA.

Article Snippet: pENTER eukaryotic plasmids expressing p62 and small interfering RNA (siRNA) of p62 were purchased from Vigene Biosciences, Inc. (Rockville, MD, USA) and Shanghai GenePharma Co., Ltd. (Shanghai, China), respectively.

Techniques: Expressing, Transfection, Control, Western Blot, Cell Counting, Standard Deviation, Small Interfering RNA